Nikon Ti-Eclipse inverted microscope with TIRF unit and QuantEM EMCCD camera
Lasers
Objectives
| Magnification | Pixel size (nm) | Numerical Aperture | Working Distance (mm) | Immersion | Type |
| 10x | 430 | 0.45 | 4.00 | dry | Plan APO DIC |
| 60x | 111 | 1.45 | 0.13 | oil | Pan APO TIRF |
| 100x | 67 | 1.49 | 0.12 | oil | Plan APO TIRF |
| 100x | 67 | 1.40 | 0.13 | oil | Plan APO VC |
Camera
EM CCD camera: QuantEM512C 512x512pixels 16bit (Photometrics)
Extra info
Cell culturing equipment: Small chamber water bath incubator with CO2 regulation (Tokai Hit)
TIRF mount for manual or mechanic TIRF angle control
FRAP3D unit: ultrafast by galvo mirror positioned laserbleaching (ROPER)
Hardware autofocus: Perfect Focus System (Nikon)
Dual colour imaging with DV2 Beamsplitter (MAG Biosystems) Green/Red or Cyan/Green
Metamorph Imaging Software
Applications
Live cell TIRF/WideField imaging under CO2 and 37oC conditions
Membrane studies (in vitro or in vivo)
Dynamic studies with Fluorescence Recovery After Photobleaching (FRAP)
Multichannel imaging
Single Molecule Imaging, PALM and STORM
Imaging activatable or switchable fluorophores
Fixed cell imaging